SITE DIRECTED MUTAGENESIS
\sˈa͡ɪt da͡ɪɹˈɛktɪd mjˌuːte͡ɪd͡ʒˈɛnɪsˌɪs], \sˈaɪt daɪɹˈɛktɪd mjˌuːteɪdʒˈɛnɪsˌɪs], \s_ˈaɪ_t d_aɪ_ɹ_ˈɛ_k_t_ɪ_d m_j_ˌuː_t_eɪ_dʒ_ˈɛ_n_ɪ_s_ˌɪ_s]\
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Mutagenesis where the mutation is caused by in vitro induction directed at a specific site in a DNA molecule. The most common method involves use of a chemically synthesized oligonucleotide mutant which can hybridize with the DNA target molecule. The resulting mismatch-carrying DNA duplex may then be transfected into a bacterial cell line and the mutant strands recovered.
By DataStellar Co., Ltd
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